1467 CREATINE KINASE BRAIN ISOENZYME CONCENTRATION CKBB IN CEREBROSPINAL FLUID(CSF) OF NEWBORNS
نویسندگان
چکیده
منابع مشابه
Raised concentration of plasma creatine kinase BB isoenzyme in myelodysplasia.
A 72 year old woman presented with a suspected myocardial infarction. An echocardiograph showed no acute changes but her plasma creatine kinase (CK) activity was increased at 343 U/l (< 175 normal range). The apparent creatine kinase-MB activity by a CK-M subunit immunoinhibition assay was 350 U/l. In view of the discrepancy between the total creatine kinase and CK-MB activity plasma creatine k...
متن کاملMeasurement of brain-specific creatine kinase isoenzyme activity in serum.
We have modified the columti-chromatographic technique of Mercer (1) to produce a sensitive and rapid separation of serum CK2 and CK3, even at normal total serum CK activity (3).12 However, CKj was not separated from CK2. Here, we report a modification of our method such that all three CK isoenzymes are separated by stepwise elution from a mini-column of diethylaminoethyl-cellulose. CK1 has onl...
متن کاملMuscle creatine kinase isoenzyme expression in adult human brain.
Previous studies have suggested that MM creatine kinase is a muscle-specific protein and is not present in adult brain tissue. We have isolated a protein from human brain with an apparent molecular weight of 43,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis which is identical to the muscle M creatine kinase isoenzyme subunit at all 30 sequenced amino acid residue...
متن کاملCreatine Kinase in Cerebrospinal Fluid.
The estimation of serum adenosine triphosphatcreatine phosphotransferase (C.P.K.) or creatine kinase has been proved valuable in the diagnosis of myopathy and in myocardial infarction (Dreyfus, Schapira, and Demos, 1960a; Dreyfus, Schapira, Scebat, Renais, and Lenegre, 1960b). As well as muscle tissue, the brain has been shown to contain a high enzyme concentration (Narayanaswami, 1952; Colombo...
متن کاملCreatine kinase isoenzymes in human cerebrospinal fluid and brain.
Extracts of normal brains obtained at autopsy and cerebrospinal fluid (CSF) from patients with global brain ischemia were analyzed for creatine kinase (CK; EC 2.7.3.2) isoenzymes. We used both qualitative and quantitative assays (electrophoresis and immunoinhibition). Brain extracts contained CK-BB isoenzyme and mitochondrial CK. In 54 CSF samples free of blood contamination and with total acti...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1981
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-198104001-01496